40ft. These primers are then used to amplify the STR fragment from 10 different elephants. 11. The use of the flanking DNA sequences around each restriction site is an important aspect of RAD tags. Of course you didn’t really expect a simple answer did you? Answer: very STR has multiple alleles, or variants, each defined by the number of repeat units present or by the length of the sequence. We confirm the important role of flanking sequences using binding assays. for a forty stack, I do 20 Infantry, 8 Cavalry, and 12 Artillery. 10. Explain your answer. The 5′ flanking region of the human GR was first isolated and characterized by Zong et al. Chloroplast genome sequences are essential to build transgene cassettes because they provide both flanking and regulatory sequences. Would you expect the fragment to be the same size in all the elephants? Why are flanking sequences important for amplifying STR fragments? A scientist makes primers specific to a particular STR fragment in elephants. 10. Transgene cassettes that are inserted into bacterial plasmids are called chloroplast vectors and they are bombarded into plant cells using gold particles and a gene gun (Fig. These primers are then used to amplify the STR fragment from 10 different elephants. Computer analysis of the sequence indicated that, similar to the androgen receptor gene, no TATA or CAAT boxes are present in the promoter region of the GR gene. Highly divergent N‐ and C‐terminal flanking sequences are important for the function of sHsps, as the isolated α‐crystallin domains investigated so far show no chaperone activity (22 – 24). (57). Why are flanking sequences important for amplifying STR fragments? ... Flanking regions are important because knowing their sequences enables geneticists to isolate the STR using polymerase chain … 3b). 11. The density of RAD tags in a genome depends on the restriction enzyme used during the isolation process. 30ft. of movement is enough to run almost a complete circle around a medium creature, a size of creature most players are expected to face most of the time. The structures show that the amino acids N-terminally flanking the basic cluster make specific contacts with the receptor that are distinct from the interactions between bipartite NLSs and Impα. Starting from a limited amount of known sequence to identifying the sequences flanking it, is a challenge relevant to many analyses (Table 1). Would you expect the fragment to be the same size in all the elephants? This is actually an important point. Isolation of RAD tags. This is really a fascinating question of tactics and the correct answer depends on what armies in what time period under what leadership are facing one another! That is why you need a good army composition. A genomic clone of about 3.0 kb was isolated and sequenced. 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